Chapter 4. Discovery of new beauty value - AMORE STORIES - ENGLISH
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2016.09.29
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Chapter 4. Discovery of new beauty value

Asian Beauty
Lab

The story of Asian Beauty Lab, dedicated to discovering and recreating Asian beauty


Chapter 4. Discovery of new beauty value :
soybean from the dining table to cosmetics

WRITER
Kim Donghyun

The topic of Asian Beauty Lab's fourth column is the soybean, one of the Amorepacific Heritage ingredients. The soybean is a food consumed daily by Korean people in various forms, such as kongbap (cooked rice with beans), soy milk, bean sprouts, tofu, soy sauce, soybean paste, and hot pepper paste. In this column, you will learn about the research findings on the soybean carried out by Amorepacific R&D Center. This column was written with the great help of Kang Younggyu, who is solely in charge of soybean research at the Heritage Material Research Team.

"Soybeans! What Are They and Where Are They from?"

Peas, kidney beans, lentils, chickpeas, peanuts – most of these edible seeds and fruits are categorized as 'legumes,' a class of vegetables that includes beans, peas and lentils. The soybean, which is also known by its scientific names of 'Glycine max' and 'Glycine soja,' is generally referred to as '菽 (suk)', '豆 (du)', or '太 (tae)' in hanja (Korean name for Chinese characters). The soybean is a type of legume that is traditionally called 'daedu,' 'baektae,' or 'seoritae' in Korea.
  • Classification of Legumes

Although the hanja '菽 (suk)' is not commonly used today, you may well know the term 'Sukmaek,' which refers to a person lacking judiciousness or a baby in the woods. 'Sukmaek' literally means soybeans and barley. The English word 'soybean' comes from the introduction of soy sauce in the West from Japan in the 1600s, and it refers to the type of legume used to make soy sauce. The word was born by combining 'soyu' — the Japanese pronunciation of soy sauce — with 'bean'. As the etymology of 'soybean' indicates, the soybean is a plant first introduced to the West in the 1600s, which was about 400 years ago today.

Then, where does the soybean come from? A comprehensive consideration of historical records, ancient relics, and the plant's natural habits shows that the soybean came from Northeast Asia, which refers to today's Korean peninsula, Manchuria, and the Primorsky Krai region of Eastern Russia. Of the world's five most important crops, namely soybeans, wheat, rice, corn, and barley, only soybeans are originally from the Korean Peninsula, a fact which highlights their importance in Korean history.
  • Place of origin, dissemination route, and main producing areas of soybeans

As mentioned earlier, the internationalization of the soybean occurred relatively recently. The soybean was first introduced to Europe around 1690 and to America around 1770. While it has only been 100 years or so since soybeans were first grown on the American continent, the region is now the world's largest soybean producer.

That then begs the question: why did the West take to the soybean? The answer is because of some of the natural characteristics and features of the soybean. With regard to composition, the soybean has the highest percentage of protein content of all crops, and it contains abundant oil. With regard to cultivation, the soybean lives in symbiosis with nitrogen-fixing bacteria. As a consequence, it protects the soil it grows on, enhancing the soil's fertility. The soybean is therefore conducive to sustainable agriculture and environmental preservation more generally.

The soybean, whose excellence is recognized by easterners and westerners alike! The soybean, one of the world's five most important crops and the only one originating from Korea! Now, don't you feel proud of and special about the soybean a little?

The Soybean, a Staple of Korean Food Culture

Ancient mural depicting jangyang (藏釀)

Cultivated for thousands of years in Korea, the soybean is both a crop and food, and for that reason, it has become inseparable from Korean food culture and features prominently on the dining tables of Korean people. For Korean people, the soybean has long been the main source of protein, which would otherwise be lacking in a traditional diet. In addition, using soybean-fermented foods such as soybean paste and soy sauce, Korean people were able to enrich the flavors of other Korean foods, while also significantly improving their storability. Tofu, which is coagulated soy protein, and bean sprouts, which are made by germinating soybeans, are widely used in a variety of Korean cuisine. In terms of utility, it seems safe to say that there isn't any food ingredient more important to Korean food culture than the soybean.

Of all the techniques associated with culinary culture to use soybeans, what Korean people excel at more than anything else is the art of making soybean-fermented foods. This argument is supported by historical records of the Three Kingdoms period of Korea. In many history books documenting the period such as The Story of Goguryeo (高句麗傳) from The Book of Liang (梁書) and The Nansi (南史), and The Book of Wei : The Story of Dong Yi (魏志東夷傳) from The Records of the Three Kingdoms (三國志), there are accounts saying that the people of Goguryeo liked to keep things clean and were good at jangyang (藏釀). Here, jangyang is a general term referring to fermented foods and related processes, including brewing liquor, making soybean-fermented foods (soybean paste, hot pepper paste, soy sauce), and pickled vegetables (kimchi). The historical accounts indicate that Korean people have been excellent at making soybean-fermented foods since at least the Goguryeo period. Such an approach to the soybean differs widely from that of the West, where the soybean was traditionally considered an industrial crop used mainly for its protein and oil content, which in turn demonstrates the ingenuity and excellence of Korean food culture.

# The Nascent Period of Soybean Research (1986–1994)

Starting with a patent filed in 1986, the first research conducted by Amorepacific R&D Center focused on soybean saponins, which are capable of improving existing saponins' skin safety, increasing human hair's tensile strength and giving it luster. In other words, Amorepacific began research into methods for producing saponin ether that can be used more efficiently in hair products such as shampoos by virtue of an increased affinity for human hair, which can be achieved by giving a cationic feature to soybean saponins.

The 1991 patent on hair growth promoting compositions followed research on hair growth promoting the benefits of medicinal extracts, which include black beans, Cornus mas, false daisy, Salvia miltiorrhiza, Sanguisorba officinalis Linne, and Semen Cuscutae. As evidenced by the content of the two patents above, in the initial research, Amorepacific took a great interest in developing soybean-originated materials with human hair-related effects. Amorepacific has been interested in soybean-based beauty research since more than thirty years ago, and such interest spurred us on to begin our soybean research in earnest in the 1990s.

# The Growth Period of Soybean Research (1994–2010)

Fermentation of Bacillus subtilis

It would be accurate to say that 1994 was when Amorepacific set out to discover new beauty values in the soybean. The research conducted at that time led to the discovery of 'soypol,' which is a material still widely used today as a natural moisturizing substance. In 1990, there weren't any respectable cosmetics manufacturer in Korea, and most Korean companies used raw materials imported from Japan. Of those raw materials, a fermented soybean polymer called γ-PGA produced by a manufacturer particularly caught our attention. γ-PGA is a white-thread-like substance that stretches like a cobweb in cheonggukjang (fermented soybean paste). It is a large natural polymer containing an amino acid called glutamic acid.

γ-PGA is a natural substance that's highly effective at moisturizing the skin, and yet, in Korea in the 1990s, there wasn't any manufacturer capable of producing it. We made the localization of the substance our mission and set out to conduct joint research with Amorepacific's Ansan Biochemistry Division. Back then, developing a fermentation technique based on single microbial strains without the problem of microbial contamination and establishing a purification process for producing high-purity γ-PGA required a considerable level of technological capability. After about two years of hard work and numerous trials and errors, we finally obtained — for the first time in Korea — a technique necessary for resolving our most persistent problems, such as contamination due to other microbial strains and the taste change of raw materials. The newly developed raw material was named 'soypol,' and its name evolved into 'soypol Plus'. Soypol is a compound word of 'soy,' which means a soybean, and 'pol(y),' which means a polymer. The fact that it's still widely used today as a natural moisturizing substance for skincare products makes soypol more than qualified to become the first material of our soybean-originated heritage.

Mamonde Total Solution Moisture Cream

The next thing we will be looking at is our research on isoflavone, the most well-known of all bioactive substances after the soybean. Isoflavone is a substance commonly found in legumes. In soybeans, isoflavone exists in the form of a glycoside, which is where glucose is bonded to daidzein and genistein. However, compared to the glycoside form which contains glucose, the aglycon type is free of glucose and is vastly superior when it comes to its skincare effectiveness. Amorepacific thus began research to produce aglycon-type isoflavone. By developing a technique for manufacturing an isoflavone aglycon rid of glucose with the use of microbial fermentation in 1999, we succeeded in developing a new raw material called 'bio-isogen', which contains high levels of soybean isoflavone aglycon. After verifying isoflavone aglycon's effectiveness at reducing skin wrinkles and skin tightening, Amorepacific filed a patent in 2003 on composition for externally applied cosmetics containing isoflavone aglycon. This 'bio-isogen' material was then applied to Mamonde Total Solution Cream.

The next fruit of Amorepacific's soybean research is ODI, which was applied to HYOSIAH as a key benefiting substance. ODI is short for Ortho-DihydroxyIsoflavone, which is a substance created by optionally adding the –OH chemical structure to isoflavone. There's an interesting story to the discovery of this substance: one day, Amorepacific's researchers found out that some cancer patients specifically ate soybean paste that had been fermented for at least 5 years for the treatment of their cancer. The researchers became curious as to what unique substances a 5-year-old soybean paste contained, and after searching every corner of Korea, they eventually procured a medical-purpose soybean paste that had been fermented for more than 5 years. The results from compositional analysis of the soybean paste showed that it contained a substance called ODI that was not found in ordinary soybean paste. Further research revealed that ODI is created from microbial fermentation in soybeans and that isoflavone changes to ODI at a very slow rate.

In addition, research conducted on the skin-related efficacy of ODI showed that ODI has far higher levels of effectiveness for anti-oxidizing, brightening, and anti-aging than ordinary soybean isoflavone. Such ODI research results were published in 7 SCI papers, making the research study the one that resulted in the highest number of papers among all single-topic research studies conducted by Amorepacific. ODI can only be obtained from soybean pastes fermented for 5 years or longer, and such old soybean pastes are indeed rare to find. To commercialize ODI as a raw material in skincare products, Amorepacific began searching for a microbe that could accelerate isoflavone's change to ODI and optimize fermentation techniques that used the microbe. Following these developments, Amorepacific embarked on the development of soybean-fermented materials containing ODI. As of 2016, Amorepacific's ODI research is centered on developing techniques capable of obtaining regular amounts of high-purity ODI, and the company plans to apply the outcome of the research to developing its future soybean cosmetic products.
  • HYOSIAH Oneum Line released in 2010


# The Maturity Period of Soybean Research (2011–2016)

The soybean research we've looked at so far has focused on methods for processing the soybean. However, as Amorepacific's soybean research has entered its maturity period, the focus has moved on to the soybean itself as a plant, a stage that precedes research on processing processes. First off, let's take a look at the story behind the discovery of Beauty Bean No.1, the flat bean.

As explained earlier, the soybean spread all around the world from the Korean peninsula and Manchuria. Recently published papers have revealed that Korea is the original home of the largest variety of wild beans, cementing Korea as the undisputed origin of beans. Think of the wild beans mentioned here as the ancestors of the beans we grow and use today.

Due to the difficulty of cultivation, wild beans are not mass-produced for commercial use. They have nevertheless been a Beauty Bean contender for a long time because of the superior levels of skin-benefiting substances they contain. However, wild beans' low productivity makes them scarce and difficult to obtain. Amorepacific thus turned to the Rural Development Administration to procure more than 140 types of native Korean bean. After going through a rigorous selection process based on such things as cultivation specificity, compositional analysis, and skin benefits, Amorepacific went on to conduct research on suitable beauty materials. Consequently, of the 140 types of bean, the flat bean, so called for its flat shape, was selected as AP Beauty Bean No.1. After a further three years of optimizing its cultivation techniques, Amorepacific became able to mass produce it in quantities fit for commercial use.
  • AP Beauty Bean Garden: research for the restoration of Korean native beans took place in here.

  • Korean native beans in a storage container

  • Comparison of the flat bean and the soybean

The winning flat bean contains high levels of epicatechin, an anti-oxidant substance not found in other types of cultivated bean. With its strong anti-oxidant property, this substance is known to prevent the degradation of skin collagen. Research also reveals that when flat beans are germinated, their amino acid levels increase six-fold. Taking full advantage of the flat bean's compositional characteristics, Amorepacific has been able to develop Wild-firmingTM Complex in 2016 by balancing flat bean epicatechin-rich materials with anti-oxidized germination extracts. This new raw material has been since applied to primera Wild Seed Firming Cream, which is released in September.
  • primera Wild Seed Firming Cream

Research for the restoration of the flat bean is highly significant in terms of biodiversity. In the current circumstances where people only grow general types of beans suitable for an agricultural environment dominated by mass production which prioritizes ease of cultivation and harvesting, pest resistance, and productivity, the fact that Amorepacific is able to once again grow Korea's precious inherited resource – the flat bean – which differs significantly from other cultivated beans and has been able to commercialize it proves that our continuing research has contributed to bean diversity.

Amorepacific is currently farming AP Beauty Bean No.2, which is derived from the flat bean but offers a more excellent composition and greater ease of cultivation. The new Beauty Bean is expected to be commercialized in 2019.

Rediscovery of Bean Leaves

Amorepacific built its Beauty Bean Garden in 2011 and has conducted bean cultivation research there ever since. In the process, Amorepacific took a significant interest in bean leaves, which it had overlooked previously. This makes sense because, unlike bean seeds, bean leaves are typically exposed to the external environment for 4-5 months at a time, making them highly likely to contain more varied bioactive substances than bean seeds do. Amorepacific had to find out which growth stage's bean leaves were the best in terms of skin benefits. Amorepacific sampled bean leaves at each growth stage, with compositional analysis of the bean leaves revealing that those harvested when turned yellow possess properties most beneficial to the skin.
  • Bean plant at different growth stages

  • Bean leaves that have changed color

Additionally, to find out what kind of cultivation environment is the best for beans to produce leaves with more excellent beneficial effects, Amorepacific grew the same type of bean on Jeju and Yeongwol, two wildly different cultivation environments, and assessed the beneficial effects of the bean leaves. The results of the assessment revealed that the bean leaves harvested on Jeju offer greater beneficial effects. The bean leaves were then grown and harvested by Amorepacific in cooperation with OSULLOC Farm, with Amorepacific succeeding in the raw materialization of substances refined from yellowed bean leaves that contain high levels of coumestrol, which has been proven to deliver excellent anti-aging effects.
  • Cultivation and harvesting of yellowed bean leaves

The substances refined from yellowed bean leaves were then applied to Sulwhasoo's Goa line products, and the findings from the research won MAFRA (Ministry of Agriculture, Food and Rural Affairs)'s New Technology Certification as 'technology that has commercialized cosmetics made from bean leave extracts containing high levels of coumestrol'.
  • Sulwhasoo Goa Cream

  • MAFRA's New Technology Certification

Amorepacific is the first company in the world to have developed raw materials for cosmetics based on bean leaves. This is an innovative development where Amorepacific didn't merely reduce a plant called bean to just its seed form, but rather saw it as a plant and studied it accordingly.

"The Soybean, a Food Valued by the UN"

The UN has declared 2016 the International Year of Pulses. Following 2014's International Year of Family Farming and 2015's International Year of Soils, this is the third agriculture-related international year declared by the UN. Why did the UN choose the soybean?

The soybean is a nutritionally balanced food not only because 20-45% of it consists of protein, but also because it contains various beneficial substances such as carbohydrates, vitamins, and minerals. Moreover, the bacteria that live in symbiosis with the roots of the soybean secure nitrogen from the air and enrich the soil. Thanks to its high tolerance to drought, the soybean is considered key to solving the human-caused food crisis resulting from climate change, population growth, and rising meat consumption.

The soybean has been recognized as the most important crop for the sustainable development of mankind and the environment! Don't you agree that soybean research must go on, not only for the sustainable development of Amorepacific, but also for the sustainable development of Korea? Amorepacific would like to ask for your continued interest and support in helping us conduct more meaningful and fruitful research on the soybean as our proud heritage ingredient. Thank you for reading!


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